Clinical performance of exigo™ H400 hematology analyzer compared with reference instrument

A CBC is highly useful in general screenings as a tool to aid in diagnosis and monitoring of disease conditions. Automated instruments for this type of analyses were developed as early as in the 1950s, with the first European cell counter developed by Erik Ohlin, the founder of the human Swelab Instruments (now part of Boule Diagnostics). Boule launched their first veterinary system 2004.

Before, blood cell counts were performed manually by microscopy. Although manual examination of blood smears is still used as a control method for verification of results from abnormal samples, the automated hematology analyzers have largely replaced the manual method for determination of

hematology parameters in routine use. Automated analyzers can also provide much information outside of what a manual microscopy can give you for certain parameters.

exigo H400 automated 4-part hematology analyzer.

Figure 1. exigo H400 automated 4-part hematology analyzer.

The exigo H400 system is an automated hematology analyzer for in vitro diagnostic use under laboratory conditions (Fig. 1). The analyzer is intended for determination of hemoglobin (HGB) concentration, for counting of red blood cells (RBC) and platelets (PLT), as well as for counting and differentiation of white blood cells (WBC) into four subpopulations, namely lymphocytes (LYM), monocytes (MONO), neutrophils (NEU), and eosinophils (EOS). The measurement principles of the exigo H400 are based on impedance for cell counts and spectrophotometry for HGB.

This study validates the performance of exigo H400 against a reference system for dog, cat and horse, using normal and abnormal fresh whole blood samples collected from patients for routine analysis.

Comparison of test and reference systems

The performance of the exigo H400 4-part hematology analyzer (test system) was compared to that of the Sysmex™ XT-2000iV 5-part hematology analyzer (reference system). Exigo H400 operates with impedance technology whereas the reference analyzer uses fluorescence flow cytometry. The results from this study demonstrate that the performance of exigo H400 hematology analyzer is in good agreement with that of the reference analyzer and the manual reference method, indicating the suitability of exigo H400 for use in routine hematology analysis at veterinary clinics.



Figure 1. Agreement between the test and the reference systems for dog samples. Passing-Bablok regression graphs for RBC (A), HGB (B), MCV (C), HCT (D), PLT (E), WBC (F), LYM (G), and GRAN (H). In regression plots, the gray line corresponds to identity (x = y) and the red line corresponds to best fit.

Learn more – Download the full application note or visit the product page for exigo H400 hematology analyzer.